Journal: JCI Insight
Article Title: EZH2 deletion does not affect acinar regeneration but restricts progression to pancreatic cancer in mice
doi: 10.1172/jci.insight.173746
Figure Lengend Snippet: Histological and quantitative analysis comparing KRAS G12D and KRAS G12D Ezh2 ΔSET mice 51 days after initiating KRAS G12D and 35 days after treatment with saline or cerulein. ( A ) Representative H&E images of pancreatic tissue. Box plots indicate the amount of lesion area as a percentage of the entire pancreatic tissue. Significance was measured by 1-way ANOVA followed by Tukey’s post hoc tests. ( B ) Representative IHC for CK19 or amylase followed by counterstaining with hematoxylin in cerulein-treated mice. Box plots compare the ratio of CK19 + /amylase + tissue. Significance was measured by 2-tailed unpaired Mann-Whitney U test. ( C and D ) Representative images of alcian blue histology ( C ) or periodic acid–Schiff (PSA) ( D ) histology showing advanced lesions (arrows) in saline- or cerulein-treated KRAS G12D and KRAS G12D Ezh2 ΔSET mice. Box plots compare the stained area as a percentage of ADM/PanIN lesions. Significance was measured by 2-tailed unpaired Mann-Whitney U test. Scale bar: 100 μm. For graphs, individual mice ( n = 7 mice per group) are shown and data represent mean ± minimum to maximum. * P ≤ 0.05, ** P ≤ 0.01.
Article Snippet: Slides were counterstained with hematoxylin (Biocare Medical, CATHE-M) and imaged using Leica Microscope DM5500B (Leica Microsystems) and LAS V4.4 software.
Techniques: Saline, MANN-WHITNEY, Staining